Schedule of Lab Topics
The basic laboratory schedule is as follows, but there may be some changes during the semester.
| Lab Number |
Date |
Lab exercises |
| 1 |
Mon/Tues
8/27-8/28 |
No Labs |
| 2 |
Wed/Thurs
8/29-8/30 |
Laboratory introduction, Lab safety
Microscopes (hay infusion) |
| 3 |
Mon/Tues
9/3-9/4 |
Labor Day- No Labs |
| 4 |
Wed/Thurs
9/5-9/6 |
Culture media preparation, Ch. 2 |
| 5 |
Mon/Tues
9/10-9/11 |
Inoculation of media; Aseptic technique
Bacteria:Nomemclature, Colony and Cell morphology |
| 6 |
Wed/Thurs
9/12-9/13 |
Stains: Negative Stain, Ch. 6
Capsular stain, Ch. 7
Smear preparation, Ch.8
Simple stain, Ch. 9
|
| 7 |
Mon/Tues
9/17-9/18 |
Gram stain, Ch. 10 |
| 8 |
Wed/Thurs
9/19-9/20 |
Spore Stain, Ch.11
Acid fast Stain, Ch. 12 |
9
|
Mon/Tues
9/24-9/25 |
Environmental influences: Temperature, Ch. 13
Osmolarity, Ch. 17 |
| 10 |
Wed/Thurs
9/26-9/27 |
Environmental influences: Lethal Effects of UV, Ch.14
Oxygen Tolerance, Ch. 15
pH, Ch. 16 |
| 11 |
Mon/Tues 10/1-10/2 |
Antimicrobials:Antiseptic & Disinfectants, Ch. 19
Antibiotic susceptibility, Ch. 20 |
| 12 |
Wed/Thurs
10/3-10/4 |
Fungi, Ch. 21, Lichens and Filamentous bacteria |
| 13 |
Mon/Tues
10/8-10/9 |
Algae and Cyanobacteria, Ch. 2 |
| 14 |
Wed/Thurs
10/10-10/11 |
Protozoa, Ch. 22
Review for Midterm |
| 15 |
Mon/Tues
10/15-10/16 |
MIDTERM |
| 16 |
Wed/Thurs
10/17-10/18 |
Bacteriophage. Ch. 30 |
| 17 |
Mon/Tues
10/22-10/23 |
Bacterial population count, Ch. 23 |
| 18 |
Wed/Thurs
10/24-10/25 |
Bacteria in Food and Soil, Ch. 24 |
| 19 |
Mon/Tues
10/29-10/30 |
Biochemical Test: IMVC, Ch.32
Motility, Ch.34
Sugar Fermentations, Ch. 33
Urea |
| 20 |
Wed/Thurs
10/31-11/1 |
UNKNOWN ORGANISMS ASSIGNED, Ch. 36
Oxidasem Ch. 15
Catalase, Ch. 15
Starch test |
| 21 |
Mon/Tues
11/5-11/6 |
Identification of Unknown bacteria |
| 22 |
Wed/Thurs
11/7-11/8 |
Identification of Unknown bacteria |
| 23 |
Mon/Tues
11/12-11/13 |
Epidemiology, Ch. 4 |
| 24 |
Wed/Thurs
11/14-11/15 |
Bacteriological Examination of water
Unknown report due |
| 25 |
Mon/Tues
11/19-11/20 |
Transformation Ch. 28
Conjugation, Ch. 29 |
| 26 |
Wed/Thurs
11/21-11/22 |
Thanksgiving - No Labs |
| 27 |
Mon/Tues
11/26-11/27 |
Rapid Plasmid Isolation, Ch. 31 |
| 28 |
Wed/Thurs
11/28-11/29 |
Gel Electrophoresis
Microbial Interrelationship
Review for final |
| 29 |
Mon/Tues
12/3-12/4 |
FINAL EXAM |
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Lab Syllabus
Lab Manual
Beck, Hughes and O’Donovan, Microorganisms, A Laboratory Manual
Please bring your lab manual to each laboratory session.
Attendance
Microbiology laboratory meets ftwice a week (as assigned, either Monday and Wednesday or Tuesday and Thursday). Attendance to lab is mandatory.
Note that additional time to work is on "Odentification of Unknown Assignment" will be required between the dates of 10/29-11/13.
Quizzes, Assignment and Exams
Quizzes are administered in the first 10 minutes of class. Arriving late to lab after a quiz has been administered will automatically result in a grade of “ZERO” on the quiz.
There are two practical exams (midterm and final) The practical midterm exam will be administered halfway through the semester and a non-comprehensive, practical final exam will be administered at the end of the semester. Exam questions may include short answers, fill-in-the-blank, multiple choice, short answers, identification and practical questions.
There will be no laboratory quizzes or exam make-ups. Quizzes or Exam grades will not be dropped.
Academic Dishonesty
Anyone caught cheating on any quiz or exam will receive a 0% and notification will be sent to the Dean of Students.You can be dropped from the class with a failing grade for plagiarism and/or cheating.
Note that additional time outside scheduled lab times will be required in order to complete your unknown assignment.
Composition of Lab Grade:
| Quizzes (4) |
20% |
| Midterm Exam |
30% |
| Final Exam |
30% |
| Unknown report |
10% |
| Attendance/lab reports |
10% |
Lab Policies- See “SAFETY RULES” for additional lab policies.
• If you miss a lab, you are responsible for the information covered that day.
•All work is done independently unless explicitly instructed otherwise.
•Students are responsible for leaving their workstations clean and orderly.
•Students may leave the lab only after checking out with his/her lab instructor.
• Note that additional time outside schedule lab times will be required in order to complete your "Unknown
Identification Assignment"
• It is the student's responsibility to be aware of all University of North Texas policies and dealines for dropping courses or withdrawing.
POLICY ON DISABILITIES ACCOMODATION
The Department of Biological Sciences complies with the American with Disabilities Act in making reasonable accommodations for qualified students with disabilities. If you have a qualifying disability as defined by the ADA and would like to request accommodations, please see the instructor by the 4th day of class.
Professor:
Gerard A. O’Donovan
Lab coordinator:
Roxana Hughes
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Lab Meeting Times
Location: Biology Building, Room 228
Days meeting: Monday through Thursday
Sections:
301 M&W 1:00PM-2:50 PM
Instructor:
302 M&W 3:00PM-4:50 PM
Instructor:
303 T &R 1:00PM-2:50 PM
Instructor:
304 T&R 3:00PM-4:50 PM
Instructor:
305 M&W 5:00PM-6:50 PM
Instructor:
306 T&R 5:00PM-6:50 PM
Instructor:
307 T&R 7:30AM-9:20 PM
Instructor:
309 M&W 7:00PM-8:50 PM
Instructor:
313 M&W 11:00AM-12:50 PM
Instructor:
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Assignments
N/A
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Safety Rules
SAFETY RULES
The following list of safety regulations must be followed without exception.
- Absolutely no eating, drinking, smoking or applying cosmetics in the laboratory!
- Always wash your hands before and after completing your laboratory work.
- Leave hats, coats, books, bags and other items on the coat and book- rack. Cluttered walkways and lab benches are dangerous.
- Do not place anything in your mouth that has come in contact with infectious materials.
- No open toed shoes, i.e. no sandals, flip-flops, slides, etc
- Report all injuries and/or accidents immediately to the instructor, no matter how small, there are no penalties for accidents, provided they are reported promptly.
- Dispose of biohazard materials properly. Disposable and broken glass items should be placed in the designated glass box. All other trash should be placed in trash cans. Used test tubes should be placed in the designated area.
- Spills of bacterial cultures- Wear gloves, mop up with bleach 5%. Dispose in
autoclave bag. Wash your hands, call for the instructor.
- Broken glassware- Must not be handled directly by hands; use brush and dustpan
- Pippeting- When pipetting, use rubber pipette fillers or safety pipette pumps
Do not “mouth”-pipette!
- Always replace the caps on reagents, solution bottles and bacterial cultures
- Pay attention to warnings by your instructor
- Wipe down lab bench with disinfectant before and after each lab period to minimize contamination
- Aseptic Burners are a fire hazard; long hair must be pulled back to avoid burn hazards.
- Familiarize yourself with locations of eye-wash station and sinks
- Fire extinguisher is located by door entrance.
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Related Links
N/A
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