Schedule of Lab Topics
| Date |
Laboratory Exercises |
Mon/Tues
8/27-8/28 |
No Labs |
Wed/Thurs
8/29-8/30 |
Laboratory introduction, Lab safety
Introduction to microscopes: How to see microorganisms, Ex. 2 |
Mon/Tues
9/3/-9/4 |
Labor Day- No Labs |
Wed/Thurs
9/5-9/6 |
Lab Safety quiz
Isolation of Bacteria, Ex. 6
Practice microscopy |
Mon/Tues
9/10-9/11 |
Simple Stain, Ex. 2
Gram Stain, Ex. 2
Bacteria nomenclature |
Wed/Thurs
9/12-9/13 |
The growth of Bacteri: Bacterial count, Ex. 8 |
Mon/Tues
9/17-9/18 |
Effect of Temperature on Bacterial growth |
Wed/Thurs
9/19-9/20 |
Control of Microorganisms: Antiseptic and Disinfectants |
Mon/Tues
9/24-9/25 |
Microbial Control: The Phenol Coefficient Test, Ex. 29 |
Wed/Thurs
9/26-9/27 |
Metabolic Properties of Bacteria, Ex. 10 |
Mon/Tues
10/1-10/2 |
Algae and Protozoa, Ex. 3 |
Wed/Thurs
10/3-10/4 |
Fungi, Ex. 4
Metazoan Parasites, Ex. 15 |
Mon/Tues
10/8-10/9 |
Water availability (aw) Ex. 30
Osmolarity |
Wed/Thurs
10/10-10/11 |
The Enteric Bacteria, Ex. 11
Examination of Chicken Flesh for Samonella, Ex. 12 |
Mon/Tues
10/15-10/16 |
MIDTERM EXAM
Turn in notebooks |
Wed/Thurs
10/17-10/18 |
Biochemical Test for Identification of Bacteria |
Mon/Tues
10/22-10/23 |
Most probable Number of coliforms in water, Ex. 21 |
Wed/Thurs
10/29-10/30 |
Decection of Fecal Contamination of Food and water |
Mon/Tues
10/30-11/1 |
Fermentation: Beer Production, Ex. 29 |
Wed/Thurs
11/1-11/2 |
Presence of Toxin-prodcuting bacteria in Food |
Mon/Tues
11/5-11/6 |
Standard Plate count of milk |
Wed/Thurs
11/7-11/8 |
Fermentation: Production of Yogurt, Ex. 27 |
Mon/Tues
11/12-11/13 |
Genetically modified Foods |
Wed/Thurs
11/14-11/15 |
Genetically modified Foods, continued |
Mon/Tues
11/19-11/20 |
Epidemiology Experiment |
Wed/Thurs
11/21-11/22 |
Thanksgiving-No Labs |
Mon/Tues
11/26-11/27 |
Alkaline Phosphatease, Ex. 23
Review for final Exmination |
Wed/Thurs
11/28-11/29 |
Final Exam
Turn in notebooks |
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Lab Syllabus
Laboratory manual:
Microbiology Laboratory for Food Sciences by G.R. Vela
NOTE: Do not buy a used laboratory manual
Attendance:
Attendance is mandatory. If you miss a lab, you are responsible for the information covered that day. There are no make-up labs. If you are absent more than twice during the semester, you may be academically dropped with the grade of WF for non-attendance.
Quizzes and Exams
Quizzes are administer in the first 10 minutes of class. Arriving late to lab after a quiz has been administered will automatically result in a grade of “ZERO” on the quiz.
There will be no laboratory quiz or exam make-ups.
Notebooks
Notebooks are daily assignment related to the experiment that are conducted in the labs. They may consist of any combination of the following:
- Drawing and sketches
- Questions to be answered located at the end of each lab exercise
- Demonstration of skills or identification of structures
Late notebooks will be penalized 10 percent per day late. (late work is anything turned in after the lab period ends on the due date)
Students must be present in order to complete daily assignments.
Lab Policies
Students must follow all safety rules and guidelines for the laboratory.- See “Safety Rules” for additional lab policy information. Failure to comply may result in removal from the laboratory and the assignment of an absence for that lab period.
All work is to be done independently unless explicitly instructed otherwise. You can be dropped from the class with a failing grade for plagiarism and/or cheating. You can also be dropped for three or more unexcused absences. Students more than 10 minutes late to lab will be considered absent for that day for attendance grade. It is the student’s responsibility to be aware of all university policies and deadlines for dropping courses or withdrawing.
POLICY ON DISABILITIES ACCOMODATION
The Department of Biological Sciences complies with the American with Disabilities Act in making reasonable accommodations for qualified students with disabilities. If you have a qualifying disability as defined by the ADA and would like to request accommodations, please see the instructor by the 4th day of class.
Composition of lab grade:
| Laboratory manual |
20% |
| Attendance/Participation |
10 % |
| 4 Quizzes |
20 % |
| Midterm Lab Exam |
25% |
| Final Lab Exam |
25% |
Release of Grades
The Family Educational Rights and Privacy Act (FERPA) (1974) does not permit faculty or staff to report grades by phone or e-mail.
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Lab Meeting Times
LOCATION: Biology Building, Room 258
DAYS MEETING: Applied Microbiology Laboratory meets Monday - Thursday. Attendance is mandatory.
SECTIONS:
Section 501
M&W
1:00PM- 2:20PM
Instructor (TBA)
Section 502
T&R
8:00am- 9:20am
Instructor (TBA)
Section 503
T&R 6:00PM-7:20PM
Instructor: (TBA)
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Assignments
N/A
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Safety Rules
SAFETY RULES
The following list of safety regulations must be followed without exception.
All students must sign an acknowledgement of Lab safety training form in order to work in the Applied Micro labs.
- Absolutely no eating, drinking, smoking or applying cosmetics in the laboratory!
- Spills of bacterial cultures -Wear disposable gloves. Mop up with
Hypochlorite (Bleach 5%) Dispose into autoclave bag. Wash hands.
Call for the instructor, there are no penalties for accidents, provided they
are reported promptly.
- Splashes on face or in eyes- Call for the instructor! Know where the
Emergency Eye-Wash is located
- Report all injuries and/or accidents immediately to the instructor,
no matter how small they are.
- Broken glassware- Do not open leaking or broken specimen containers.
Broken glassware must not be handled directly by hands; use brush and dustpan
- Pipetting- When pipetting, use rubber pipette fillers or safety pipette pumps
Never “mouth”-pipette!
- Always replace the caps on reagents, solution bottles and bacterial cultures
- Always wash your hands before and after completing your laboratory work
- Dress- No open toed shoes, i.e. no sandals, flip-flops, slides, etc.
Always wear laboratory coat and disposable gloves when working in this lab
Eye protection and mask should be worn when conducting experiments that create splashes
or aerosols
- Dispose of biohazard materials properly, do not throw refuse of any kind into the sink
- Bleach your bench before and after each experiment
- No unauthorized person is allowed in the Medical Bacteriology Laboratory.
- The inoculating needle should be heated until red-hot before and after use. Always flame
needle before you lay it down
- Aseptic Burners are a fire hazard; long hair must be pulled back to avoid burn hazards.
- Familiarize yourself with location of eye-wash station and sinks
- Fire extinguisher is located by door entrance
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Related Links
N/A
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