Name:  Kent D. Chapman

Phone:  (940) 565-2969 or 2301
FAX:  (940) 565-4136
E-MAIL:  chapman@unt.edu

Address:

Department of Biological Sciences
Division of Biochemistry and Molecular Biology
University of North Texas
PO Box 305220
Denton, TX 76203-5220

1983-1986 Lycoming College, Williamsport, PA, A.B. (Biology)

1986-1991 Arizona State University, Tempe, AZ, Ph.D. (Botany), Dissertation Title: "The Cellular Origin of Membrane Lipids for Enlarging Cottonseed Glyoxysomes", Supervisory Professor: Dr. Richard N. Trelease

1986-1991 Graduate Research or Teaching Assistant/Associate Arizona State University, Tempe

1989-1990 Summer Instructor, Biology for Majors, Arizona State University, Tempe

1991-1993 Postdoctoral Fellow, National Science Foundation Postdoctoral Fellowship in Plant Biology, Louisiana State University, Baton Rouge, LA, Advising Professor: Dr. Thomas S. Moore, Jr. Project Title: "Biosynthesis of N-Acylphosphatidyl-ethanolamine"

1993-1998 Assistant Professor of Biochemistry, Department of Biological Sciences, Division of Biochemistry and Molecular Biology, University of North Texas, Denton, TX 76203

1998-2003 Associate Professor of Biochemistry, Department of Biological Sciences, Division of Biochemistry and Molecular Biology, University of North Texas, Denton, TX 76203

2003-present Professor of Biochemistry, Department of Biological Sciences, Division of Biochemistry and Molecular Biology, University of North Texas, Denton, TX 76203

1999-2003 Director, Biochemistry and Molecular Biology Division, Department of Biological Sciences, University of North Texas, Denton, TX 76203

2003-present Director, Center for Plant Lipid Research, College of Arts and Sciences, University of North Texas, Denton, TX   76203

2001-2002 Visiting Scientist, Plant Biology Division, Samuel R. Noble Foundation, Ardmore, OK   73402.   Sabbatical/ Developmental Leave- Laboratory of Dr. Richard A. DixonHonorary Societies

• Beta Beta Beta National Biology Society
• Phi Sigma Tau National Philosophy Society
• Phi Kappa Phi National Honor Society
• Society for Sigma Xi
• American Society for Biochemistry and Molecular Biology
• American Oil Chemists Society
• American Society of Plant Biologists (formerly American Society of Plant Physiologists)
• American Association for the Advancement of Science
• National Plant Lipid Cooperative

Blancaflor, E.B. and Chapman, K.D. (2006) Similarities between endocannabinoid signaling in animal systems and N-acylethanolamine metabolism in higher plants. In Communication in Plants, F. Baluska, S. Mancuso, and D Volkmann eds., Ch. 14, Springer-Verlag, pp 205-219.

1. Chapman, K.D., Turley, R.B., Trelease, R.N. 1989. Relationship between cottonseed malate synthase aggregation behavior and suborganellar location in glyoxysomes and endoplasmic reticulum. Plant Physiol. 89(1): 352-359.
2. Chapman, K.D. and Trelease, R.N. 1990. Inhibition of cottonseed choline- and ethanolaminephosphotransferases by calcium during postgerminative growth. Plant Physiol. 93(4): 1525-1529.
3. Chapman, K.D. and Trelease, R.N. 1991. Intracellular localization of phosphatidylcholine and phosphatidylethanolamine synthesis in cotyledons of cotton seedlings. Plant Physiol. 95(1): 69-76.
4. Chapman, K.D. and Trelease, R.N. 1991. Acquisition of membrane lipids by differentiating glyoxysomes-- role of lipid bodies. J. Cell Biol. 115(4): 995-1007.
5. Chapman, K.D. and   Trelease, R.N. 1992. Characterization of the membrane proteins of enlarging cottonseed glyoxysomes. Plant Physiol. and Biochem. 30 (1): 1-10.
6. Chapman, K.D. and Moore, T.S.,Jr. 1993. N-acylphosphatidylethanolamine synthesis in plants: occurrence, molecular composition, and phospholipid origin. Arch. Biochem. Biophys. 301: 21-33.
7. Chapman, K.D. and Moore, T.S.,Jr. 1993. Catalytic properties of a newly-discovered acyltransferase that synthesizes N-acylphosphatidylethanolamine in cottonseed (Gossypium hirsutum L.) microsomes. Plant Physiol. 102(3): 761-769.
8. Chapman, K.D. and Moore, T.S.,Jr. 1994. Isozymes of cottonseed microsomal N-acylphosphatidylethanolamine synthase: Detergent solubilization and electrophoretic separation of active enzymes with different properties. Biochim. Biophys. Acta, 1211: 29-36.
9. Cai, S.J., McAndrew, R.S., Leonard, B.P., Chapman, K.D., and Pidgeon, C. 1995. Rapid purification of cotton seed membrane-bound N-acylphosphatidylethanolamine synthase by Immobilized Artificial Membrane (IAM) chromatography. J. Chromatography A, 696: 49-62.  
10. McAndrew, R.S., Leonard, B.P., and Chapman, K.D. 1995. Photoaffinity labeling of cottonseed microsomal N-acylphosphatidylethanolamine synthase protein with a substrate analogue, 12-[(4-azidosalicyl)amino]dodecanoic acid. Biochim. Biophys. Acta, 1256: 310-318.  
11. Chapman, K.D., Lin, I., and Desouza, A.D. 1995. Metabolism of cottonseed microsomal N-acylphosphatidylethanolamine. Arch. Biochem. Biophys., 318: 401-407.  
12. Maier, C.G.-A., Chapman, K.D., and Smith, D.W. 1995. Estrogenic activity of female plant extracts from two dioecious species. Plant Science, 109: 31-43.
13. Chapman, K.D., Jackson, A.C., Moreau, R.A., and Tripathy, S. 1995. Increased N-acylphosphatidylethanolamine biosynthesis in elicitor-treated tobacco cells. Physiologia Plantarum, 95:120-126.
14. Sandoval, J.A., Huang, Z-H., Garrett, D.C., Gage, D.A., and Chapman, K.D. 1995. N-Acylphosphatidylethanolamine in dry and imbibing cotton (Gossypium hirsutum L.) seeds: amounts, molecular species and enzymatic synthesis. Plant Physiology,109: 269-275.  
15. Chapman, K.D. and Sprinkle, W.B. 1996. Developmental, tissue-specific, and environmental factors regulate the biosynthesis of N-acylphosphatidylethanolamine in cotton (Gossypiumhirsutum L.). Journal of Plant Physiology 149: 277-284.  
16. Chapman, K.D., McAndrew, R.S., and Huynh, T.T. 1997. Cottonseed calnexin: identification and isolation of a membrane-bound molecular chaperone. Plant Physiology and Biochemistry 35(6): 483-490.  
17. Maier, C.G.-A., Chapman, K.D., and Smith, D.W. 1997. Phytoestrogens and floral development in dioecious Maclura pomifera (Raf.). Plant Science 130(1):27-40.
18. Chapman, K.D., and Sriparameswaran, A. 1997. Intracellular localization of N-acylphosphatidylethanolamine synthesis in cotyledons of cotton seedlings. Plant and Cell Physiology; 38(12): 1359-1367.  
19. Hemphill, J.K., Maier, C.G.A., Chapman, K.D. 1998. Rapid in-vitro plant regeneration of cotton (Gossypium hirsutum L.). Plant Cell Reports,17(4): 273-279.  
20. McAndrew, R.S., and Chapman, K.D. 1998. Enzymology of cottonseed N-acylphosphatidylethanolamine synthase: kinetic properties and mechanism-based inactivation Biochimica Biophysica Acta 1390: 21-36.  
21. Chapman, K.D., Tripathy, S., Venables, B.J., Desouza, A.D. 1998. N-Acylethanolamines: formation and molecular composition of a new class of plant lipids. Plant Physiology, 116: 1163-1168.
22. Pappan, K., Austin-Brown, S., Chapman, K.D., Wang, X. 1998. Substrate selectivities and lipid modulation of plant phospholipase Dalpha, beta, and gamma. Arch. Biochem. Biophys. 353: 131-140.  
23. Chapman, K.D. 1998. Phospholipase activity during plant growth and development and in response to environmental stress. Trends in Plant Science, 3(11): 419-426. (Review)
24. Pirtle, R.M., Huynh, T.T., Yoder, D., Nampaisansuk, M., Pirtle, I., Chapman, K.D. (1999) Characterization of a palmitoyl-acyl carrier protein thioesterase (FatB1) in Cotton (Gossypium hirsutum L.). Plant Cell Physiology 40: 155-163.
25. Chapman, K.D. , Venables, B., Markovic, R.M., Blair, R., Bettinger, C. (1999) N-Acylethanolamines in seeds: quantification of molecular species and their degradation upon imbibition. Plant Physiology 120: 1157-1164.
26. Hoang, C.V., Wessler, H.G., Local, A., Turley, R.B., Benjamin, R.C., Chapman, K.D. (1999) Identification and expression of cotton (Gossypium hirsutum L.) plastidial carbonic anhydrase. Plant Cell Physiology 40(12):  
27. Yoder, D.W., Nampaisansuk, M., Pirtle, I. Chapman, K.D., Pirtle R.M. (1999) Molecular cloning and nucleotide sequence of a gene encoding a cotton palmitoyl-acyl carrier protein thioesterase. Biochimica et Biophysica Acta, 1446: 403-413.
28. Tripathy, S., Venables, B., Chapman, K.D. (1999) N-Acylethanolamines in elicitor signal transduction: Attenuation of alkalinization and activation of defense gene expression. Plant Physiology 121: 1299-1308.
29. Morefield, S.I., Keefer, E.W., Chapman, K.D., Gross, G.W. (2000) Drug evaluations using neuronal networks on microelectrode arrays: characteristic effects of cannabinoid agonists anandamide and methandamide on cortical and spinal cultures. Biosensors and Bioelectronics 15:383-396.
30. Petersen, G., Chapman, K.D., Hansen, H.S. (2000) A rapid phospholipase D assay using zirconium precipitation of anionic substrate phospholipids: application to N-acylethanolamine formation in vitro. Journal of Lipid Research41(9): 1532-40.
31. Chapman, K.D. (2000) Emerging physiological roles for N-acylphosphatidylethanolamine metabolism in plants: membrane protection and signal transduction. Chemistry and Physics of Lipids 108: 221-230. (Review)
32. Chapman, K.D. , Austin-Brown, S. Sparace, S.A., Kinney, A.J., Ripp, K.G., Pirtle, I.L, Pirtle, R.M. (2001) Transgenic cotton plants with increased seed oleic acid content. Journal of the American Oil Chemists’ Society (JAOCS) 78(9): 941-947.
33. Pirtle, I., Kongcharoensuntorn, W., Nampaisansuk, M., Knesek, J., Chapman K.D., Pirtle R.M. (2001) Molecular cloning and functional expression of the gene for a cotton delta-12 desaturase (FAD2) Biochimica et Biophysica Acta 1522(2): 122-129.
34. Huynh, T., Pirtle, R.M., Chapman, K.D. (2002) Expression of a Gossypium hirsutum cDNA encoding a FatB palmitoyl-acyl carrier protein thioesterase in Eschericia coli. Plant Physiology and Biochemistry, 40: 1-9.
35. Hoang, C.V. and Chapman, K.D. (2002) Biochemical and molecular inhibition of plastidial carbonic anhydrase reduces the incorporation of acetate into lipids in cotton embryos and tobacco cell suspensions and leaves.  Plant Physiology 128 (April): 1417-1427.
36. Hoang, C.V. and Chapman, K.D. (2002) Regulation of plastidial carbonic anhydrase gene expression in cotton (Gossypium hirsutum, L) seedlings during post-germinative growth. Plant Molecular Biology 49: 449-458.
37. Austin-Brown, S. and Chapman, K.D. (2002) Inhibition of phospholipase Dalpha by N-acylethanolamines. Plant Physiology 129: 1892-1898.
38. Shrestha, R., Noordemeer, M., Van der Stelt, M., Veldink, G.A., and Chapman, K.D. (2002) N-Acylethanolamines are metabolized by lipoxygenase and amidohydrolase in competing pathways during cotton (Gossypium hirsutum, L.) seed imbibition. Plant Physiology 130: 391-401.
39. Chapman, K.D., Venables, B.J., Dian, E.E. and Gross, G.W.   (2003) Identification and quantification of neuroactive N-acylethanolamines in cottonseed processing fractions. Journal of the American Oil Chemists’ Society (JAOCS),80(3): 223-229.
40. Tripathy, S., Kleppinger-Sparace, K., Dixon, R.A., and Chapman, K.D. (2003) N-Acylethanolamine signaling in tobacco is mediated by a membrane-associated, high affinity binding protein. Plant Physiology, 131: 1781-1791.
41. Blancaflor, E.B., Hou, G., and Chapman, K.D. (2003) Elevated levels of N-lauroylethanolamine, an endogenous constituent of desiccated seeds, disrupt normal root development in Arabidopsis thaliana seedlings. Planta, 217: 206-217.
42. Shrestha, R., Dixon, R.A., Chapman, K.D. (2003) Molecular Identification of a Functional Homologue of the Mammalian Fatty Acid Amide Hydrolase in Arabidopsis thaliana. Journal of Biological Chemistry 278(37): 34990-34997.
43. Chapman, K.D. (2004) Occurrence, metabolism and prospective functions of N-acylethanolamines in plants. Progress in Lipid Research 43: 302-327.
44. Venables, B.J., Waggoner, C.A., Chapman, K.D. (2005) N-Acylethanolamines in Selected Legumes. Phytochemistry 66:1913-1918.
45. Motes, C.M., Pechter , P., Yoo, C.M., Wang, Y.S., Chapman,K.D., Blancaflor, E.B (2005) Differential effects of two phospholipase D inhibitors, 1-butanol and N- acylethanolamine (NAE), on in vivo cytoskeletal organization and Arabidopsis seedling growth. Protoplasma, 226:109-123.
46. Wanjie, S.W., Welti, R., Moreau, R.A., Chapman, K.D. (2005) Identification and Quantification of Glycerolipids in Cotton Fibers: Reconciliation with Metabolic Pathway Predictions from DNA Databases. Lipids, 40(8):773-785.
47. Shrestha, R., Kim, S.-C., Dyer, J., Dixon, R.A., Chapman, K.D. (2006) Plant Fatty Acid (Ethanol) Amide Hydrolases. Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1761(3):324-334 and on-line-doi:10.1016/j.bbalip.2006.03.004 .
48. Wang Y-S, Shrestha, R., Wiant, W., Venables, B.J., Chapman, KD., Blancaflor, E.B. (2006) Manipulation of Arabidopsis Fatty Acid (Ethanol) Amide Hydrolase Expression Leads to Plants with Modified Growth Characteristics and Altered Sensitivity to N-Acylethanolamines. Proceedings of the National Academy of Sciences, 103(132): 12197-12202.
49. Binns, D., Januszewski, T., Chen, Y., Hill, J., Markin, V., Zhao, Y., Gilpin, C, Chapman, K.D., Anderson, R.G.W., Goodman, J.M. (2006) Pexopodia and Gnarls, Novel Structures that Indicate an Intimate Collaboration Between Peroxisomes and Lipid Bodies. Journal of Cell Biology, 173: 719-731.
50. Welti R, Shah J, Li W, Li M, Chen J, Burke JJ, Fauconnier ML, Chapman K, Chye ML, Wang X (2007) Plant lipidomics: discerning biological function by profiling plant complex lipids using mass spectrometry. Front Biosci 12:2494-2506.
51. Bartz R, Li WH, Venables B, Zehmer JK, Roth MR, Welti R, Anderson RG, Liu P, Chapman KD (2007) Lipidomics reveals adiposomes store ether lipids and mediate phospholipid traffic. J Lipid Res 48(4):837-47.
52. Bartz R, Seemann J, Zehmer JK, Serrero G, Chapman KD, Anderson RG, Liu P (2007) Evidence that mono-ADP-ribosylation of CtBP1/BARS regulates lipid storage. Mol Biol Cell 18(8):3015-25.
53. Kilaru A, Blancaflor EB, Venables BJ, Tripathy S, Mysore KS, Chapman KD (2007)The N-acylethanolamine-mediated regulatory pathway in plants. Chem Biodivers 4(8):1933-55. Review.
54. Teaster ND, Motes CM, Tang Y, Wiant WC, Cotter MQ, Wang YS, Kilaru A, Venables BJ, Hasenstein KH, Gonzalez G, Blancaflor EB, Chapman KD (2007) N-Acylethanolamine metabolism interacts with abscisic acid signaling in Arabidopsis thaliana seedlings.
    Plant Cell 19(8):2454-69.

• Faculty Supervisor, Electron Microscopy Facility, Department of Biological Sciences, UNT 94-99
• Coordinator, UNT Department of Biological Sciences Fall Seminar Series 1999, 2000, 2002
• Chair, UNT Department of Biological Sciences, Faculty Search Committee 1999/00 (Biochemistry)
• Member, UNT Department of Biological Sciences, Faculty Search Committee 2002/03 (Biochemistry)
• Member, UNT Department of Biological Sciences, Faculty Search Committee 2002/03 (Environmental Chemistry)
• Director, Biochemistry and Molecular Biology Division, member of Departmental Executive Committee, 1999-Present (Except Fall 2001 when on developmental leave)
• Ad-hoc reviewer on research grant applications to the National Science Foundation, U.S. Dept of Agriculture, National Science and Engineering Research Council (Canada), and the U.S. Department of Energy
• Ad-hoc reviewer of manuscripts submitted to Plant Physiology, Plant Cell, Plant Journal, Trends in Plant Sciences, Biochimica et Biophysica Acta, Nature (Cell Biology), Proceedings of the National Academy of Sciences, the Journal of Lipid Research, Lipids, Plant Physiology and Biochemistry, the Journal of Plant Physiology, Agronomy Journal, Journal of Agriculture and Food Chemistry.
• Invited Lecturer, Plant Biochemistry Summer Course (NSF, DOE, USDA-sponsored), July 1999, 2001 Washington State University. Signaling Role of Plant Lipids.
• Member, Texas Food and Fiber Commission, Industry Advisory Committee. (1997-present).
• Workshop Co-organizer, Keystone Symposium on Signal Perception in Biotic Interactions of Plants "Biochemical and Genetic Approaches to the Identification of Signaling Molecules" Feb 2000.
• Member, Scientific Organizing Committee, biannual meeting of the National Plant Lipid Cooperative, held June 4-8, 2003, Stanford Sierra Camp, S. Lake Tahoe, CA.
• Local Host Annual meeting of the Southern Section American Society of Plant Biologists, March 15-17, 2003, Radisson Hotel, Denton,TX.
• Panel member, USDA- National Research Initiative Competitive Grants Program, Plant Growth and Development
• Panel member, USDA- National Research Initiative Competitive Grants Program, Value-Added Products Program
• Panel member, USDA- National Research Initiative Competitive Grants Program, Plant Biochemistry
• Panel member, NSF- Program in Metabolic Biochemistry
• Assistant Editor, Journal of Cotton Science, published on-line by the National Cotton Council.
• Member, Scientific Organizing Committee, biannual meeting of the National Plant Lipid Cooperative, to be held June 10-14, 2005, Stanford Sierra Camp, S. Lake Tahoe, CA

• Regulation of N-Acylphosphatidylethanolamine (NAPE) Metabolism. United States Department of Agriculture- Agriculture Research Service. Beltsville, MD, Sept 30, 1996.
• Regulation of N-Acylphosphatidylethanolamine Metabolism. Department of Botany, University of Texas, Austin, TX, October 10, 1996.
• An Unusual Phospholipid (NAPE) in Cottonseeds. Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX, Nov 4, 1996.
• N -Acylphosphatidylethanolamine Metabolism and Signal Transduction in Plant Defense. Salk-Noble Foundation Annual Plant Biology Retreat. October 23, 1998. Palm Springs, CA.
• N -Acylphosphatidylethanolamine Metabolism and Signal Transduction in Plant Defense. Swedish Biotechnology Network Meeting. November 11, 1998. Umea, Sweden.
• N -Acylphosphatidylethanolamine Metabolism in Plants. Department of Pharmacology, Medical College of Wisconsin. April 29, 1999.
• Regulation of Lipid Metabolism in Plants. Department of Biological Sciences, University of North Texas, Environmental Sciences Seminar Series, Spring 1999.
• N- Acylethanolamines in Elicitor Perception. Keystone Symposium, Signals and Signal Perception in Plant-Biotic Interactions, Feb 2000, Taos, NM.
• N- Acylethanolamines in Plants. Consumer Products Sector, Monsanto Co. St.Louis MO, July 1999.
• Modification of Cottonseed Fatty Acid Composition. Biology Department, Midwestern State University. Wichita Falls, TX. Dec 6, 2000.
• Topics in Plant Lipid Metabolism: seed oils, lipid mediators, and human health. Genetics Seminar Series, Texas A&M University, College Station, TX.  Jan 24, 2002
• Metabolism of N-Acylethanolamines in Plants: A New Group of Membrane-Derived Lipid Mediators. Samuel R. Noble Foundation, Plant Biology Division, Ardmore, OK, Feb 4, 2002
• Concepts in Plant Development:  Comparative Developmental Physiology Roundtable Forum. Glen Rose, TX, June 3, 2002
• Natural Sources of Neuroactive N-Acylethanolamines. American Osteopathic Association, National Convention, Las Vegas, NV, Oct 8, 2002.
• Identification and Characterization of a Binding Protein for N-Acylethanolamines. 15 th International Symposium on Plant Lipids, Okazaki, Japan, May 16, 2002
• N -Acylethanolamines: a new group of lipid mediators in plants. Biochemistry and Molecular Biology Department Seminar Series, Oklahoma State University, Stillwater OK, Nov 22, 2002.
• Metabolism of N-Acylethanolamines in Plants: A New Group of Membrane-Derived Lipid Mediators. Midwestern State University, Biology Department, Wichita Falls, TX, April 2003
• Functional Identification of an Arabidopsis thaliana N-Acylethanolamine Amidohydrolase. National Plant Lipid Cooperative- Symposium on the Biochemistry and Molecular Biology of Plant Fatty acids and Glycerolipids, June 7, 2003, Fallen Leaf Lake, CA
• Identification and Accumulation of Neuroactive N-Acylethanolamines in Oilseeds.   226 th American Chemical Society National Meeting, Division of Agriculture and Food Chemistry—Session: Biochemistry for Designing Industrial Crops.   Sept 10, 2003, New York, NY
• Metabolic Engineering of Cottonseed Oil. Midwestern State University, Spring 2004 (Departmental Seminar Series), Wichita Falls, TX.
• Metabolic Engineering of Cottonseed Oil. Brookhaven College, Spring 2004 (Evening Series), Dallas County Community College, Dallas TX
• Regulation of N-Acylethanolamine Metabolism in Seedlings. 16 th International Symposium on Plant Lipids, Budapest, Hungary, June 4, 2004.
• Occurrence, metabolism and emerging functions of N-acylethanolamines in plants. University of Texas, Molecular Cell and Developmental Biology Series, Austin, TX, Oct. 2004
• Occurrence, metabolism and emerging functions of N-acylethanolamines in plants. Midwestern State University, Wichita Falls, TX, February 2005
• Occurrence, metabolism and emerging functions of N-acylethanolamines in plants. University of Louisiana Lafayette, February 2005
• Lipid Composition of Adiposomes, a Lipid-Rich Organelle in Chinese Hamster Ovary Cells . Symposium on Mass Spectrometry. 96 th Annual American Oil Chemists Society Meeting, Salt Lake City, UT, May1-4, 2005.
• N- Acylethanolamine Metabolism and Seedling Development in Arabidopsis. International Symposium on Plant Lipid Signaling. Raleigh, NC, Oct 26-30, 2005.
• Production of Low-Oil Cottonseed. Beltwide Cotton Conferences. Conference on Cotton Physiology. San Antonio, TX Jan 5, 2006.