Koulen CV






Publications
  1. Hiesberger T, Gourley E, Erickson A, Koulen P, Ward CJ, Masyuk TV, Larusso NF, Harris PC, Igarashi P.  Proteolytic cleavage and nuclear translocation of fibrocystin is regulated by intracellular Ca2+ and activation of protein kinase C. J Biol Chem. 2006 Nov; 281(45): 34357-64.
    PMID: 16956880 [PubMed - indexed for MEDLINE]
  2. Mafe OA, Gregg EV, Medina-Ortiz WE, Koulen P.  Localization of inositol 1,4,5-trisphosphate receptors in mouse retinal ganglion cells. J Neurosci Res. 2006 Oct; (): .
    PMID: 17061256 [PubMed - indexed for MEDLINE]
  3. Tanaka M, Duncan RS, McClung N, Yannazzo JA, Hwang SY, Marunouchi T, Inokuchi K, Koulen P.  Homer proteins control neuronal differentiation through IP(3) receptor signaling. FEBS Lett. 2006 Oct; (): . PMID: 17064693 [PubMed - indexed for MEDLINE]
  4. Stokely ME, Hwang SY, Hwang JY, Fan B, King MA, Inokuchi K, Koulen P.  Polycystin-1 can interact with homer 1/Vesl-1 in postnatal hippocampal neurons. J Neurosci Res. 2006 Oct; (): . PMID: 17016857 [PubMed - indexed for MEDLINE]
  5. Xin H, Yannazzo JA, Duncan RS, Gregg EV, Singh M, Koulen P.  A novel organotypic culture model of the postnatal mouse retina allows the study of glutamate-mediated excitotoxicity. J Neurosci Methods. 2006 Jul; 281(45): . PMID: 16876874 [PubMed - indexed for MEDLINE]
  6. Jastrow H, Koulen P, Altrock WD, Kroger S.  Identification of a beta-dystroglycan immunoreactive subcompartment in photoreceptor terminals. Invest Ophthalmol Vis Sci. 2006 Jan; 47(1): 17-24. PMID: 16384939 [PubMed - indexed for MEDLINE]
  7. Koulen P.  Pkd2l2.  AfCS-Nature Molecule Pages. 2005 Oct; doi:10.1038/mp.a001840.01(): .
  8. Duncan RS, Hwang SY, Koulen P.  Effects of Vesl/Homer proteins on intracellular signaling. Exp Biol Med (Maywood). 2005 Sep; 230(8): 527-35. PMID: 16118402 [PubMed - indexed for MEDLINE]
  9. Koulen P, Duncan RS, Liu J, Cohen NE, Yannazzo JA, McClung N, Lockhart CL, Branden M, Buechner M.  Polycystin-2 accelerates Ca2+ release from intracellular stores in Caenorhabditis elegans. Cell Calcium. 2005 Jun; 37(6): 593-601. PMID: 15862350 [PubMed - indexed for MEDLINE]
  10. Koulen P, Wei J, Madry C, Liu J, Nixon E.  Differentially distributed IP3 receptors and Ca2+ signaling in rod bipolar cells. Invest Ophthalmol Vis Sci. 2005 Jan; 46(1): 292-8. PMID: 15623787 [PubMed - indexed for MEDLINE]
  11. Koulen P, Liu J, Nixon E, Madry C.  Interaction between mGluR8 and calcium channels in photoreceptors is sensitive to pertussis toxin and occurs via G protein betagamma subunit signaling. Invest Ophthalmol Vis Sci. 2005 Jan; 46(1): 287-91. PMID: 15623786 [PubMed - indexed for MEDLINE]
  12. Wen Y, Yang S, Liu R, Perez E, Yi KD, Koulen P, Simpkins JW.  Estrogen attenuates nuclear factor-kappa B activation induced by transient cerebral ischemia. Brain Res. 2004 May; 1008(2): 147-54. PMID: 15145751 [PubMed - indexed for MEDLINE]
  13. Wen Y, Yang S, Liu R, Brun-Zinkernagel AM, Koulen P, Simpkins JW.  Transient cerebral ischemia induces aberrant neuronal cell cycle re-entry and Alzheimer's disease-like tauopathy in female rats. J Biol Chem. 2004 May; 279(21): 22684-92. PMID: 14982935 [PubMed - indexed for MEDLINE]
  14. Yang SH, Liu R, Perez EJ, Wen Y, Stevens SM, Valencia T, Brun-Zinkernagel AM, Prokai L, Will Y, Dykens J, Koulen P, Simpkins JW.  Mitochondrial localization of estrogen receptor beta. Proc Natl Acad Sci U S A. 2004 Mar; 101(12): 4130-5. PMID: 15024130 [PubMed - indexed for MEDLINE]
  15. Westhoff JH, Hwang SY, Duncan RS, Ozawa F, Volpe P, Inokuchi K, Koulen P.  Vesl/Homer proteins regulate ryanodine receptor type 2 function and intracellular calcium signaling. Cell Calcium. 2003 Sep; 34(3): 261-9. PMID: 12887973 [PubMed - indexed for MEDLINE]
  16. Hwang SY, Wei J, Westhoff JH, Duncan RS, Ozawa F, Volpe P, Inokuchi K, Koulen P.  Differential functional interaction of two Vesl/Homer protein isoforms with ryanodine receptor type 1: a novel mechanism for control of intracellular calcium signaling. Cell Calcium. 2003 Aug; 34(2): 177-84.
    PMID: 12810060 [PubMed - indexed for MEDLINE]
  17. Kaja S, Yang SH, Wei J, Fujitani K, Liu R, Brun-Zinkernagel AM, Simpkins JW, Inokuchi K, Koulen P.  Estrogen protects the inner retina from apoptosis and ischemia-induced loss of Vesl-1L/Homer 1c immunoreactive synaptic connections. Invest Ophthalmol Vis Sci. 2003 Jul; 44(7): 3155-62. PMID: 12824266 [PubMed - indexed for MEDLINE]
  18. Leite MF, Thrower EC, Echevarria W, Koulen P, Hirata K, Bennett AM, Ehrlich BE, Nathanson MH.  Nuclear and cytosolic calcium are regulated independently. Proc Natl Acad Sci U S A. 2003 Mar; 100(5): 2975-80. PMID: 12606721 [PubMed - indexed for MEDLINE]
  19. Johenning FW, Zochowski M, Conway SJ, Holmes AB, Koulen P, Ehrlich BE.  Distinct intracellular calcium transients in neurites and somata integrate neuronal signals. J Neurosci. 2002 Jul; 22(13): 5344-53. PMID: 12097486 [PubMed - indexed for MEDLINE]
  20. Koulen P, Brandstätter JH.  Pre- and Postsynaptic Sites of Action of mGluR8a in the mammalian retina. Invest Ophthalmol Vis Sci. 2002 Jun; 43(6): 1933-40. PMID: 12037002 [PubMed - indexed for MEDLINE]
  21. Koulen P, Cai Y, Geng L, Maeda Y, Nishimura S, Witzgall R, Ehrlich BE, Somlo S.  Polycystin-2 is an intracellular calcium release channel. Nat Cell Biol. 2002 Mar; 4(3): 191-7. PMID: 11854751 [PubMed - indexed for MEDLINE]
  22. Koulen P, Janowitz T, Johenning FW, Ehrlich BE.  Characterization of the calcium-release channel/ryanodine receptor from zebrafish skeletal muscle. J Membr Biol. 2001 Oct; 183(3): 155-63. PMID: 11696857 [PubMed - indexed for MEDLINE]
  23. Khan ZU, Koulen P, Rubinstein M, Grandy DK, Goldman-Rakic PS.  An astroglia-linked dopamine D2-receptor action in prefrontal cortex. Proc Natl Acad Sci U S A. 2001 Feb; 98(4): 1964-9. PMID: 11172059 [PubMed - indexed for MEDLINE]
  24. Koulen P, Janowitz T, Johnston LD, Ehrlich BE.  Conservation of localization patterns of IP(3) receptor type 1 in cerebellar Purkinje cells across vertebrate species. J Neurosci Res. 2000 Sep; 61(5): 493-9. PMID: 10956418 [PubMed - indexed for MEDLINE]
  25. Hering H, Koulen P, Kroger S.  Distribution of the integrin beta 1 subunit on radial cells in the embryonic and adult avian retina. J Comp Neurol. 2000 Aug; 424(1): 153-64. PMID: 10888745 [PubMed - indexed for MEDLINE]
  26. Koulen P, Ehrlich BE.  Reversible block of the calcium release channel/ryanodine receptor by protamine, a heparin antidote. Mol Biol Cell. 2000 Jul; 11(7): 2213-9. PMID: 10888663 [PubMed - indexed for MEDLINE]
  27. Hack I, Koulen P, Peichl L, Brandstätter JH.  Development of glutamatergic synapses in the rat retina: the postnatal expression of ionotropic glutamate receptor subunits. Vis Neurosci. 2000 Jan; 19(1): 1-13. PMID: 12180854 [PubMed - indexed for MEDLINE]
  28. Koulen P, Thrower EC.  Pharmacological modulation of intracellular Ca(2+) channels at the single-channel level. Mol Neurobiol. 2000 Jan; 24(1-3): 65-86. PMID: 11831555 [PubMed - indexed for MEDLINE]
  29. Koulen P, Honig LS, Fletcher EL, Kroger S.  Expression, distribution and ultrastructural localization of the synapse-organizing molecule agrin in the mature avian retina. Eur J Neurosci. 1999 Dec; 11(12): 4188-96. PMID: 10594644 [PubMed - indexed for MEDLINE]
  30. Koulen P.  Clustering of neurotransmitter receptors in the mammalian retina. J Membr Biol. 1999 Sep; 171(2): 97-105. PMID: 10489422 [PubMed - indexed for MEDLINE]
  31. Koulen P, Kuhn R, Wässle H, Brandstätter JH.  Modulation of the intracellular calcium concentration in photoreceptor terminals by a presynaptic metabotropic glutamate receptor. Proc Natl Acad Sci U S A. 1999 Aug; 96(17): 9909-14. PMID: 10449793 [PubMed - indexed for MEDLINE]
  32. Koulen P.  Postnatal development of GABAA receptor beta1, beta2/3, and gamma2 immunoreactivity in the rat retina. J Neurosci Res. 1999 Jul; 57(2): 185-94. PMID: 10398296 [PubMed - indexed for MEDLINE]
  33. Blank M, Koulen P, Blake DJ, Kroger S.  Dystrophin and beta-dystroglycan in photoreceptor terminals from normal and mdx3Cv mouse retinae. Eur J Neurosci. 1999 Jun; 11(6): 2121-33. PMID: 10336681 [PubMed - indexed for MEDLINE]
  34. Koulen P.  Localization of synapse-associated proteins during postnatal development of the rat retina. Eur J Neurosci. 1999 Jun; 11(6): 2007-18. PMID: 10336670 [PubMed - indexed for MEDLINE]
  35. Koulen P.  Postnatal development of dopamine D1 receptor immunoreactivity in the rat retina. J Neurosci Res. 1999 May; 56(4): 397-404. PMID: 10340747 [PubMed - indexed for MEDLINE]
  36. Hanley JG, Koulen P, Bedford F, Gordon-Weeks PR, Moss SJ.  The protein MAP-1B links GABA(C) receptors to the cytoskeleton at retinal synapses. Nature. 1999 Jan; 397(6714): 66-9. PMID: 9892354 [PubMed - indexed for MEDLINE]
  37. Koulen P, Fletcher EL, Craven SE, Bredt DS, Wässle H.  Immunocytochemical localization of the postsynaptic density protein PSD-95 in the mammalian retina. J Neurosci. 1998 Dec; 18(23): 10136-49. PMID: 9822767 [PubMed - indexed for MEDLINE]
  38. Koulen P, Garner CC, Wässle H.  Immunocytochemical localization of the synapse-associated protein SAP102 in the rat retina. J Comp Neurol. 1998 Aug; 397(3): 326-36. PMID: 9674560 [PubMed - indexed for MEDLINE]
  39. Fletcher EL, Koulen P, Wässle H.  GABAA and GABAC receptors on mammalian rod bipolar cells. J Comp Neurol. 1998 Jul; 396(3): 351-65. PMID: 9624589 [PubMed - indexed for MEDLINE]
  40. Wässle H, Koulen P, Brandstätter JH, Fletcher EL, Becker CM.  Glycine and GABA receptors in the mammalian retina. Vision Res. 1998 May; 38(10): 1411-30. PMID: 9667008 [PubMed - indexed for MEDLINE]
  41. Brandstätter JH, Koulen P, Wässle H.  Diversity of glutamate receptors in the mammalian retina. Vision Res. 1998 May; 38(10): 1385-97. PMID: 9667006 [PubMed - indexed for MEDLINE]
  42. Koulen P, Malitschek B, Kuhn R, Bettler B, Wässle H, Brandstätter JH.  Presynaptic and postsynaptic localization of GABA(B) receptors in neurons of the rat retina. Eur J Neurosci. 1998 Apr; 10(4): 1446-56. PMID: 9749799 [PubMed - indexed for MEDLINE]
  43. Koulen P, Blank M, Kroger S.  Differential distribution of beta-dystroglycan in rabbit and rat retina. J Neurosci Res. 1998 Mar; 51(6): 735-47. PMID: 9545087 [PubMed - indexed for MEDLINE]
  44. Koulen P, Brandstätter JH, Enz R, Bormann J, Wässle H.  Synaptic clustering of GABA(C) receptor rho-subunits in the rat retina. Eur J Neurosci. 1998 Jan; 10(1): 115-27. PMID: 9753119 [PubMed - indexed for MEDLINE]
  45. Blank M, Koulen P, Kroger S.  Subcellular concentration of beta-dystroglycan in photoreceptors and glial cells of the chick retina. J Comp Neurol. 1997 Dec; 389(4): 668-78. PMID: 9421146 [PubMed - indexed for MEDLINE]
  46. Brandstätter JH, Koulen P, Wässle H.  Selective synaptic distribution of kainate receptor subunits in the two plexiform layers of the rat retina. J Neurosci. 1997 Dec; 17(23): 9298-307. PMID: 9364075 [PubMed - indexed for MEDLINE]
  47. Koulen P.  Vesicular acetylcholine transporter (VAChT): a cellular marker in rat retinal development. Neuroreport. 1997 Sep; 8(13): 2845-8. PMID: 9376516 [PubMed - indexed for MEDLINE]
  48. Koulen P, Brandstätter JH, Kroger S, Enz R, Bormann J, Wässle H.  Immunocytochemical localization of the GABA(C) receptor rho subunits in the cat, goldfish, and chicken retina. J Comp Neurol. 1997 Apr; 380(4): 520-32. PMID: 9087530 [PubMed - indexed for MEDLINE]
  49. Koulen P, Kuhn R, Wässle H, Brandstätter JH.  Group I metabotropic glutamate receptors mGluR1alpha and mGluR5a: localization in both synaptic layers of the rat retina. J Neurosci. 1997 Mar; 17(6): 2200-11. PMID: 9045744 [PubMed - indexed for MEDLINE]
  50. Koulen P, Malitschek B, Kuhn R, Wässle H, Brandstätter JH.  Group II and group III metabotropic glutamate receptors in the rat retina: distributions and developmental expression patterns. Eur J Neurosci. 1996 Oct; 8(10): 2177-87. PMID: 8921309 [PubMed - indexed for MEDLINE]
  51. Brandstätter JH, Koulen P, Kuhn R, van der Putten H, Wässle H.  Compartmental localization of a metabotropic glutamate receptor (mGluR7): two different active sites at a retinal synapse. J Neurosci. 1996 Aug; 16(15): 4749-56.
    PMID: 8764662 [PubMed - indexed for MEDLINE]
  52. Koulen P, Sassoe-Pognetto M, Grunert U, Wässle H.  Selective clustering of GABA(A) and glycine receptors in the mammalian retina. J Neurosci. 1996 Mar; 16(6): 2127-40. PMID: 8604056 [PubMed - indexed for MEDLINE]
Presentations
 Liu Z, Duncan RS, Koulen P, Wagenknecht T.  Binding of Homer Protein Induces Conformational Changes in Clamp Regions of Skeletal Muscle Ryanodine Receptor Presented at:  49th Annual Meeting of the Biophysical Society; Oct 01, 2005; Long Beach, California.
Books
 Koulen P. Pharmacology of intracellular calcium channels. In: , eds. Membrane Science and Technology Series, 7, Planar Lipid Bilayers (BLMs) and their Applications. Amsterdam, New York: Elsevier Science; 2003:Chapter 26 (pp. 723-734).
Teaching

Basic and Clinical Pharmacology (PHRM 6410)
Integrative Biomedical Sciences III (BMSC 5800)
Core Curriculum in Biomedical Sciences BMSC Core III: Physiology and Pharmacology (BMSC 5700)
Molecular Aspects of cell signaling (BIOC 5435)
Motility Journal Club (BIOC 5920)
Neurobiology of Aging (PHRM 5100)
Neuropharmacology (PHRM 5070)
Receptors: Molecular Basis of Drug Action (PHRM 6080)
Seminar in Current topics: Introduction to Faculty Research (BMSC 5940)
Intracellular Calcium Signaling (PHRM 5200)
Functional Neuroscience (PHRM 6000)
Nervous System 2 (NERV9410)


Graduate School Teaching:

Instructor in team-taught courses:
• Basic and Clniical Pharmacology (PHRM6410); 2002, 2003, 2004, 2005
• Integrative Biomedical Sciences III (BMSC5800); 2002, 2003; changed to:
• Core Curriculum in Biomedical Sciences BMSC Core III: Physiology and Pharmacology (BMSC5700); 2004, 2005, 2006
• Molecular Aspects of cell signaling (BIOC5435); 2003, 2005
• Motility Journal Club (BIOC5920); 2002, 2003
• Neurobiology of Aging (PHRM5100); 2002, 2003, 2005
• Neuropharmacology (PHRM5070); 2002, 2004
• Receptors: Molecular Basis of Drug Action (PHRM6080); 2003, 2005
Seminar in Current Topics:
•Introduction to Faculty Research (BMSC5940); 2001, 2002, 2003, 2006
Examination Coordinator:
•Core Curriculum in Biomedical Sciences (BMSC Core III: Physiology and Pharmacology (BMSC5700); 2005, 2006
Course director (courses conceived, organized and taught) and instructor:
•Intracellular Calcium Signaling (PHRM5200); 2003, 2004, 2005, 2006
• Functional Neuroscience (PHRM6000); 2004, 2005, 2006

Medical School Teaching:

Instructor in team-taught courses:
• Nervous System 2 (NERV9410); 2004
• Musculoskeletal System 1 (MSS9130); 2006
Funding
 • Texas Higher Education Coordinating Board; Advanced Research Program; Research Grant 000130-0014-2001 (PI: Peter Koulen); 01/01/02- 12/31/03; Function and regulation of polycystin-2 in C. elegans – a model for Polycystic Kidney Disease
• NIH / NIA; T32 AG020494 (PI: James W. Simpkins; Co-I: Peter Koulen); 05/01/02-04/30/07; Predoctoral training in the neurobiology of aging
• Texas Higher Education Coordinating Board; Advanced Research Program; Research Grant 000130-0014-2001 (PI: Peter Koulen); 06/10/02 - 08/02/02; Function and regulation of polycystin-2 in C. elegans – a model for Polycystic Kidney Disease, Supplemental Grant to support a high school teacher’s research project
• National Alliance for Research on Schizophrenia and Depression (NARSAD); Young Investigator Award (PI: Peter Koulen); 07/01/02- 06/30/04; Role of Homer proteins in intracellular calcium signaling - a new therapeutic target at glutamatergic synapses
• PKD Foundation; Research Grant-in-aid (PI: Peter Koulen); 01/01/03- 12/31/04; Functional Interaction of Polycystin-2 with IP3 Receptors - A Novel Mechanism underlying ADPKD
• Texas Higher Education Coordinating Board; Advanced Research Program; Research Grant 000130-0014-2001 (PI: Peter Koulen); 06/09/03 - 08/01/03; Function and regulation of polycystin-2 in C. elegans – a model for Polycystic Kidney Disease, Supplemental Grant to support a high school teacher’s research project
• NIH / NEI; R01 EY14227 (PI: Peter Koulen); 08/01/03-07/31/07; Calcium signaling in retinal neurons.
• NIH / NIA; R03 AG22163-01 (PI: Peter Koulen); 09/01/03-08/31/04; Modulation of calcium signaling by presenilin-1.
• NIH / NIA; 1P01 AG022550-01 (PI: James W. Simpkins; PI, project 3: Peter Koulen); 09/15/03-08/31/08; (budget for project 3); Mechanisms of Cognitive Decline During Aging. Project 3: Intracellular Ca2+ Signaling and Brain Aging
• NIH / NEI; R21 EY015672-01 (PI: Peter Koulen); 09/30/03-08/31/05; Lacrimal gland function – regulation by calcium signaling.
• NIH / NEI; 3R01EY014227-01A1S1 (PI: Peter Koulen); 01/04/04-12/31/06; Calcium signaling in retinal neurons; Research supplement for underrepresented minorities.
• Alzheimer’s Association; NIRG (PI: Peter Koulen); 01/01/04-12/31/06; Modulation of calcium signaling by presenilin-2.
• NIH / NIA; 2P01 AG010485-14 (PI: James W. Simpkins; PI, project 5: Peter Koulen); 03/01/05-01/31/10; (budget for project 5); Discovery of Novel Drugs for Alzheimer’s Disease. Project 5: The Role of Intracellular Ca2+ Concentration in the Development of Alzheimer’s Disease.
• NIH / NIA; 2P01 AG010485-14 (PI: James W. Simpkins; PI, core C: Peter Koulen); 03/01/05-01/31/10; (budget for core C); Discovery of Novel Drugs for Alzheimer’s Disease. Core C: Neuroimaging Core.
• NIH / NIA; 3 P01 AG010485-14S1 (PI: James W. Simpkins; PI, project 5: Peter Koulen); 09/15/2005 - 08/31/2007; Discovery of Novel Drugs for Alzheimer’s Disease. Project 5: The Role of Intracellular Ca2+ Concentration in the Development of Alzheimer’s Disease; research supplement for underrepresented minorities.
• NIH / NCRR; 1S10RR022570-01 (PI: Peter Koulen); 04/04/2006 - 04/03/2007; Confocal Microscope for Campus Expansion in the CBH building.
• Texas Higher Education Coordinating Board Advanced Research Program; Research Grant 000130-0007-2006 (PI: Peter Koulen); 05/15/06- 05/14/08; Nanotechnology sensor guided development of pharmacological treatment for neurodegenerative diseases.
• Texas Higher Education Coordinating Board; Advanced Research Program; Research Grant 000130-0007-2006 (PI: Peter Koulen); 06/05/06- 07/28/06; Nanotechnology sensor guided development of pharmacological treatment for neurodegenerative diseases. Supplemental Grant to support a high school teacher’s research project
Patents

Provisional Patent "Modulation of Intracellular Calcium Signaling by N-Acylethanolamines", U.S. PTO 60/468160, 05/06/03

full patent submitted, 05/06/04,

published on June 29, 2006 (Publication No. US-2006-0142395-A1)

Memberships
 Biophysical Society
Society for Neuroscience
Association for Research in Vision and Ophthalmology
Society of Biological Psychiatry
Awards and Honors
 • 2002-2004: Young Investigator Award, National Alliance for Research on Schizophrenia and Depression (NARSAD)
• 2002: Research Rising Star Award, Graduate School of Biomedical Sciences, University of North Texas Health Science Center at Fort Worth
• 2003: Scholar of the Society of Biological Psychiatry
• 2003: American College of Neuropsychopharmacology (ACNP) Memorial Travel Award
• 2005-2006: New Investigator Research Grant awarded by the Alzheimer’s Association
• 2006: Forty Under Forty Award, Fort Worth Business Press

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