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Figure 1: The fatty acid composition of cotton fiber (Coker 312) at different stages of development; 7,14, 21 and 28 days post anthesis (dpa). Total lipid extracts were obtained from fiber by a modified version of the Bligh and Dyer method, with addition of di17:0PC as an internal standard. Following acid catalyzed transesterification, FAMEs were analyzed by gas chromatography, and quantified based on the amount of internal standard. The relative percentages of the most common fatty acids present, is shown. Linolenate (18:3) and palmitate (16:0) were found to be the most prevalent fatty acids at all stages of fiber development. Bars represent the means and SD of three to four independent extractions.
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Profile of Polar Lipids in Elongating Cotton Fiber Cells
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Profile of Polar Lipids in Maturing Cotton Fiber Cells
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| Figure 2: Polar Lipid profiles of developing (7dpa) and mature (21dpa) Coker 312 cotton fiber cells obtained by high performance liquid chromatographic (HPLC) analysis using evaporative light scattering detection and mass spectrometry. Phospahtidylcholine (PC) was found to be the predominant lipid class in both developing and mature fiber tissue. Phosphatidylinositol (PI) was also fairly abundant, and higher levels were observed in mature fiber cells. The levels of both phosphatidylethanolamine (PE) and digalactosyl diacylglycerol (DGDG) in young fiber cells were similar to those found in mature fiber cells. Relatively low levels of phospahtidic acid (PA) and lysophospahtidylcholine (lyso PC), were observed in fiber cells. The ratio of acylated stery glycosides ASG to steryl glycosides (SG) was higher in mature fiber cells. Tissue harvest and lipid extractions were performed at UNT and Dr Robert A. Moreau, USDA-ARS-Eastern Regional Research Center, Philadelphia, PA performed the HPLC analysis. Bars represent the means and SD for three independent extractions. |
