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DNA Sequencing Core Facility | |
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University
of North Texas
Dept of Biological Sciences
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Results & Interpretation of Data The results are returned in two forms: a raw sequence file (*.seq; which can be opened as a text file) and a chromatogram file (*.ab1). If a specific file type is required for your lab software, discuss available options with either Dr. Jeff Johnson (jajohnson@unt.edu) or Angie Ambers (angie.ambers@unt.edu). When your samples have been completed, you will receive an email notification. Sequencing results will be archived in folders (labeled with the investigator’s name) on UNT's server for at least two weeks for downloading. To download result file(s) go to: www.XXXXXXXXX If your lab does not possess sofware to view the result files, it is recommended that investigators download Sequence Scanner Software v1.0 from the Applied Biosystem's website. This free software will enable you to view, edit, and print your sequence data. The sequence returned to investigators may be up to 1100 nucleotides long, but in general only some of the sequence is reliable. The amount depends on a variety of factors, but the primary determinant is the quality of the template DNA. Beyond that high-quality sequence, the remainder of the file is useful in specific experiments where sequence accuracy is unimportant. It is crucial for each customer to evaluate their own data and to determine the extent of reliable data. Because data evaluation is highly dependent on your individual needs, we cannot perform this step for you. See our page on Interpretation of Sequencing Chromatograms if you are unsure how to evaluate the results.
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