DROPOFF LOCATION IS SMALL FRIDGE IN EESAT 325E
The DNA Core Facility provides University of North Texas researchers access to high-quality automated DNA Sequencing technology on a recharge basis. The cost of this service compares favorably with similar cores at other institutions, as does the quality of the data obtained. Turnaround time is short; results are usually returned within three to five business days (M-F, 8am-5pm) starting the day after sample submission. Our turnaround time will improve as more researchers use this service because our sequencer (ABI 3130xl) runs batches of 16, and if one submits fewer than 16 samples at a time, a delay may result awaiting additional samples to obtain the number of samples required for a run. NOTE: if the data is required ASAP (e.g., to meet a fortuitous deadline) and samples are not submitted in batches of 16, please contact us and we can discuss optional payment plans. This document provides basic information needed by prospective clients of the Core. |
Description of Service
The DNA Core Facility accepts DNA samples and processes them according to protocols for Applied Biosystems DNA Sequencers (Model 3130xl). One sequencing run (one template, one primer) typically produces 900 nucleotides of high-quality sequence data or more, for good-quality template and a well-designed primer. At least one positive control (standard DNA known to generate good sequence) will be used for each sequencing run. The basic cost of DNA sequencing is $6.00 for processing one template with one primer for all clients affiliated with the University of North Texas. This cost may be adjusted in the future once we become more comfortable with the total cost required to run and maintain the equipment and facility.
**Note: Automated sequencing is very reliable. As a precaution, we always check that control samples sequenced properly, that excess terminators were removed, that the gel resolution was adequate, and that the computerized data extraction proceeded normally. If a problem is detected in any of these, customer samples are repeated free of charge. The Core Facility has no control over the quality of template, the design of the primer, or the presence of 'problem' spots on the template. We will do our best to help you avoid such problems, but we must charge for sequencing reactions that fail for such reasons.
Sample Preparation/Submission and DNA concentrations for sequencing
Helpful hints for Interpretation of Sequencing Chromatograms
contact information: Dr. Jeff Johnson (jajohnson@unt.edu) or Angie Ambers (angie.ambers@unt.edu)